| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.168484 |
| Chromosome: | chromosome 1 |
| Location: | 3411876 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021750 | (1 of 1) PF07818 - HCNGP-like protein (HCNGP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTTGTATACATGTGGGTGCACCGCGCC |
| Internal bar code: | GAGGGTTTTCCTCGCATCGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 638 |
| LEAP-Seq percent confirming: | 99.7808 |
| LEAP-Seq n confirming: | 7739 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTGTCTTTGTGTGGGCT |
| Suggested primer 2: | CTTGGAACTAACGCCTCTGC |