| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.168488 |
| Chromosome: | chromosome 5 |
| Location: | 393694 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232900 | (1 of 4) PF02181 - Formin Homology 2 Domain (FH2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACTGTTGACCACTTGACCGGATGCCGC |
| Internal bar code: | GGGTGGACGTAAATCCACCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 466 |
| LEAP-Seq percent confirming: | 99.7823 |
| LEAP-Seq n confirming: | 1375 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTGTGTGTGTGGCAGA |
| Suggested primer 2: | CATAATGCGTATGTGCGTCC |