| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.168488 |
| Chromosome: | chromosome 5 |
| Location: | 393752 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232900 | (1 of 4) PF02181 - Formin Homology 2 Domain (FH2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGCTTTTGTCCAGTTCGCCAGCATACA |
| Internal bar code: | ACGCTACGTCGATCGTGGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 967 |
| LEAP-Seq percent confirming: | 99.8196 |
| LEAP-Seq n confirming: | 1660 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTATACCGCTGCCTGCTAC |
| Suggested primer 2: | CATAATGCGTATGTGCGTCC |