| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.168502 |
| Chromosome: | chromosome 4 |
| Location: | 2786068 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223876 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCATCAACACTGCGTCAGCCCTGGCAGT |
| Internal bar code: | GTGTGGCGCTCAAAGTATTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 915 |
| LEAP-Seq percent confirming: | 99.621 |
| LEAP-Seq n confirming: | 26545 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTAAAACGCCGATTGTTG |
| Suggested primer 2: | ACGTTTTTGACCCACTCCAG |