| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168509 |
| Chromosome: | chromosome 6 |
| Location: | 7965440 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g303650 | (1 of 1) K15027 - translation initiation factor 2D (EIF2D) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGGACGCGGGAAGGACTGAATGTGGGC |
| Internal bar code: | ACACCCTCCTGAAGGGCGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 824 |
| LEAP-Seq percent confirming: | 99.4069 |
| LEAP-Seq n confirming: | 3855 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCAGAAGCAAATGGAGGA |
| Suggested primer 2: | GCTGAATCCTGCTCATGTCA |