Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.168518 |
Chromosome: | chromosome_1 |
Location: | 4117006 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g027050 | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AAATGTTTACTTGCGCAGGCAAGCAGGAGA |
Internal bar code: | TTGAATTCTTTAATTTTCTTAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1067 |
LEAP-Seq percent confirming: | 98.9302 |
LEAP-Seq n confirming: | 3514 |
LEAP-Seq n nonconfirming: | 38 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCCCAGTATCCGAAACCAAG |
Suggested primer 2: | TGCATATGGCTGTCATCGTT |