| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.168521 |
| Chromosome: | chromosome 1 |
| Location: | 7258557 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g052400 | CPLD43 | CPLD43 protein, required for cyt b6 assembly | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCTTGATGCATCAGTAAAAAAGGGGAG |
| Internal bar code: | CGGAACGTAATCTGCGCTGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 883 |
| LEAP-Seq percent confirming: | 98.7642 |
| LEAP-Seq n confirming: | 959 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAGCAAGTGCAGGAAAC |
| Suggested primer 2: | GAGCAGTTGTCGTGTGAGGA |