Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.168549 |
Chromosome: | chromosome_2 |
Location: | 7237331 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g145850 | FAP112 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTAAATTCGGCCGCGAAAGACGGCAGAAGG |
Internal bar code: | GAACCTCGGATTCACACCATCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 696 |
LEAP-Seq percent confirming: | 93.1853 |
LEAP-Seq n confirming: | 2434 |
LEAP-Seq n nonconfirming: | 178 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCCACTCCACGACACCATA |
Suggested primer 2: | GCTGAAGGCACGCTAGTACC |