| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168554 |
| Chromosome: | chromosome 10 |
| Location: | 890875 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424100 | IPY1 | (1 of 1) PTHR10286//PTHR10286:SF3 - INORGANIC PYROPHOSPHATASE // INORGANIC PYROPHOSPHATASE; Inorganic pyrophosphatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGGGACCCGGCGCCCGCCCTGGCGCTG |
| Internal bar code: | TGGATGACGGAGAGTAGCTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1081 |
| LEAP-Seq percent confirming: | 87.2821 |
| LEAP-Seq n confirming: | 851 |
| LEAP-Seq n nonconfirming: | 124 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGGGATTTTGTAGTCCCG |
| Suggested primer 2: | ATCAGCGTGTATCCACACCA |