Insertion junction: LMJ.RY0402.168582_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.168582
Chromosome:chromosome 6
Location:5230627
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g282150 CNX1G,CNX1C (1 of 2) 2.7.7.75 - Molybdopterin adenylyltransferase; Molybdenum cofactor biosynthesis protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCATGTATTGGAGCTGTCCTAGCCCTCG
Internal bar code:CGTTGTGTCTAGTTGGGGAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:359
LEAP-Seq percent confirming:99.7273
LEAP-Seq n confirming:2560
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TCAAGTCCATCCCCTACTGC
Suggested primer 2:AAACAGGTAGCCGTCAATGC