| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168585 |
| Chromosome: | chromosome 1 |
| Location: | 7674572 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g055420 | PP2A1,PP2A-B,PF4 | (1 of 1) K04354 - serine/threonine-protein phosphatase 2A regulatory subunit B (PPP2R2); Phosphatase PP2A, B subunit | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTGGGTGCCATGCGCAAGTGCGCCGGAG |
| Internal bar code: | GGGGGTGTAACGAAGAGCTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 279 |
| LEAP-Seq percent confirming: | 99.9243 |
| LEAP-Seq n confirming: | 1320 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGTTTTACATTTGCAGGT |
| Suggested primer 2: | CAACCCTTTGTATGTCGCCT |