| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168605 |
| Chromosome: | chromosome 6 |
| Location: | 5486786 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g284700 | AAT2 | Alanine aminotransferase; (1 of 1) K00814 - alanine transaminase (GPT, ALT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGGGAGAACTGGAGTCGAAACTCTTTT |
| Internal bar code: | CTACTGCCCGCTAGCGTTCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 878 |
| LEAP-Seq percent confirming: | 98.8743 |
| LEAP-Seq n confirming: | 4304 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCCGGCACTTCATACTTT |
| Suggested primer 2: | TCCTCTCAACACAACCTCCC |