| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168607 |
| Chromosome: | chromosome 2 |
| Location: | 7062536 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g147302 | (1 of 1) 2.6.1.78 - Aspartate--prephenate aminotransferase / Prephenate transaminase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGTGTGGGGTTTGGGAGGGCTGCTGTTC |
| Internal bar code: | GCTGTGGTTACGGTCCGGGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 116 |
| LEAP-Seq percent confirming: | 88.9908 |
| LEAP-Seq n confirming: | 97 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACCCAGCACGCCTAATTC |
| Suggested primer 2: | GTGCGTTGTGTATGGAATGC |