| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168621 |
| Chromosome: | chromosome 6 |
| Location: | 7303353 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298750 | AOT4 | (1 of 9) PF01490 - Transmembrane amino acid transporter protein (Aa_trans); Amino acid transporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAAACCACTGCGGCACTGCTAACGCTC |
| Internal bar code: | TCCGTCGCTCCCGGGGAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 525 |
| LEAP-Seq percent confirming: | 99.639 |
| LEAP-Seq n confirming: | 1656 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCACATTGTAACCGTTGCG |
| Suggested primer 2: | AAACTGGGAGTAGCCAGGGT |