Insertion junction: LMJ.RY0402.168636_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.168636
Chromosome:chromosome 12
Location:7947575
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g552300 CTL3 (1 of 1) IPR001304//IPR016187//IPR024616 - C-type lectin // C-type lectin fold // Pherophorin; Putative pherophorin with C-type lectin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTTGAGATCGCGCTGTACGACCACAAGG
Internal bar code:GTGTGGCGACAGGTGTAGCACT

Confirmation - LEAP-Seq

LEAP-Seq distance:356
LEAP-Seq percent confirming:86.8368
LEAP-Seq n confirming:11195
LEAP-Seq n nonconfirming:1697
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:AAACAGTAGCGCCCTCTTGA
Suggested primer 2:GAGGGAGAAGGAGAGGGAGA