| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168636 |
| Chromosome: | chromosome 13 |
| Location: | 2230594 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578550 | (1 of 3) IPR000104//IPR011009 - Antifreeze protein, type I // Protein kinase-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTCTTGTGTTGCAGCTGCTCCAGCACCT |
| Internal bar code: | CGTCGGCATGCGATGTCTTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 137 |
| LEAP-Seq percent confirming: | 42.1395 |
| LEAP-Seq n confirming: | 713 |
| LEAP-Seq n nonconfirming: | 979 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTGCCTGCCTAGATAGC |
| Suggested primer 2: | AATGGTGAGTCTGGTGAGGG |