Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.168667 |
Chromosome: | chromosome_12 |
Location: | 6142582 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g536050 | ALA1 | Phospholipid-transporting ATPase | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CACCACCATCACGGTTCGGGAGCGCACGCC |
Internal bar code: | AGATGGACAGCAGAGCCGGTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1118 |
LEAP-Seq percent confirming: | 99.7496 |
LEAP-Seq n confirming: | 5976 |
LEAP-Seq n nonconfirming: | 15 |
LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACCTTGCCATTTTGCAATC |
Suggested primer 2: | TCTCCACAATTGGGCCTATC |