| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.168675 |
| Chromosome: | chromosome 16 |
| Location: | 2092922 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657650 | FAP35 | Flagellar Associated Protein 35 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATGTAAACCCATGGGCGTCCATTGCTCA |
| Internal bar code: | CGTAATGCTATACGGTAGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 537 |
| LEAP-Seq percent confirming: | 99.5684 |
| LEAP-Seq n confirming: | 5767 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATGGGAGGGCCTGTTGTA |
| Suggested primer 2: | TACCGAACTTCTTGGGGTTG |