| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.168675 |
| Chromosome: | chromosome_16 |
| Location: | 2092924 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g657650 | FAP35 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGAGTTGCTCCGCCTGTTTGGCCAGCAGG |
| Internal bar code: | TCCGTGCGCTGGCGCACCCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1025 |
| LEAP-Seq percent confirming: | 98.2507 |
| LEAP-Seq n confirming: | 2022 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATGGGAGGGCCTGTTGTA |
| Suggested primer 2: | TACCGAACTTCTTGGGGTTG |