| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168688 |
| Chromosome: | chromosome 13 |
| Location: | 1241058 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g570400 | (1 of 3) 3.1.1.14 - Chlorophyllase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGGTGTACGCGGAATCGTGTTCAGGGC |
| Internal bar code: | ATAGTAGAGTAAACTAACGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 784 |
| LEAP-Seq percent confirming: | 98.6091 |
| LEAP-Seq n confirming: | 7373 |
| LEAP-Seq n nonconfirming: | 104 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGACGTCAAGCTACGAGG |
| Suggested primer 2: | GCTTTCGCTTGTGAATCTCC |