| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.168730 |
| Chromosome: | chromosome 14 |
| Location: | 2325085 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g623650 | ADH7 | Alcohol dehydrogenase; (1 of 3) 1.1.1.2 - Alcohol dehydrogenase (NADP(+)) / Aldehyde reductase (NADPH) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATGCGATCGAGCAGTTGGCGTACGTTT |
| Internal bar code: | GCGTGGGTCGTTTAGTTGCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 304 |
| LEAP-Seq percent confirming: | 99.8497 |
| LEAP-Seq n confirming: | 7306 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCACACCGACTTCGTACA |
| Suggested primer 2: | ACAAGTGCTGCCACTTCCTT |