| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.168732 |
| Chromosome: | chromosome 7 |
| Location: | 5928169 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g354200 | GAP2 | Glyceraldehyde 3-phosphate dehydrogenase, GAPC, cytosolic; (1 of 2) 1.2.1.12 - Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) / Triosephosphate dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGATGCTGAGGAAACAAGAAGCGCGCACG |
| Internal bar code: | TACTCTGTGCTAGCGTGGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 659 |
| LEAP-Seq percent confirming: | 99.2894 |
| LEAP-Seq n confirming: | 2096 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACAACGAGTACGGCTACA |
| Suggested primer 2: | CCCAGCACCACATACACAAC |