Insertion junction: LMJ.RY0402.168733_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.168733
Chromosome:chromosome 17
Location:5446578
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g737050 (1 of 1) PTHR22957//PTHR22957:SF287 - TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCTTCTTGTTGGCGCCGCTAGTCTCCAT
Internal bar code:CGGCCACGGCGAAAACAACCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:88
LEAP-Seq percent confirming:90.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCAATGAGGGTTATCGTG
Suggested primer 2:ACGAGATGAGGGGTGTTACG