| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.168743 |
| Chromosome: | chromosome 16 |
| Location: | 6761392 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673505 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGAGTCGTGGTGGAGGTGAGCAGCAGCGC |
| Internal bar code: | GCGAGGTGGTTTCGGAACAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 991 |
| LEAP-Seq percent confirming: | 99.745 |
| LEAP-Seq n confirming: | 1956 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAACCATGTAGCGCTGTGG |
| Suggested primer 2: | TGAGCTGATGCAGTTATGGC |