| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.168750 |
| Chromosome: | chromosome 12 |
| Location: | 1253001 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489300 | (1 of 2) IPR000742//IPR001881//IPR009030 - EGF-like domain // EGF-like calcium-binding domain // Insulin-like growth factor binding protein, N-terminal | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGTAAGGATATGGCTTGTAGCAATCACA |
| Internal bar code: | GATTCCCCCCACCAGTAGTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 445 |
| LEAP-Seq percent confirming: | 99.3506 |
| LEAP-Seq n confirming: | 765 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGAAGTTCTCTGGGCAGC |
| Suggested primer 2: | CACTGTTTGTGTGGCTTGCT |