| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.168758 |
| Chromosome: | chromosome 12 |
| Location: | 6955270 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560350 | CNK2 | (1 of 1) PTHR24361//PTHR24361:SF410 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED; NIMA-Related Kinase 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCGATCAGACGTGCTCATAAACACGCA |
| Internal bar code: | AAGATAGAAGGGTAGAGGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 497 |
| LEAP-Seq percent confirming: | 99.2271 |
| LEAP-Seq n confirming: | 4365 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTTCTGTGGTGTCCCAAG |
| Suggested primer 2: | ACAACAGCAACAACAGCAGC |