| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.168803 |
| Chromosome: | chromosome 10 |
| Location: | 5640709 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g460326 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGACACGCTTCATGTCCCTGCCTCTGGA |
| Internal bar code: | CCGAATTCTAAACCGCTTTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1182 |
| LEAP-Seq percent confirming: | 98.9222 |
| LEAP-Seq n confirming: | 1285 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCAACAGCCATCATACAA |
| Suggested primer 2: | TAAGACCATGCACCGTACCA |