Insertion junction: LMJ.RY0402.168814_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.168814
Chromosome:chromosome 10
Location:2538312
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g436700 (1 of 21) PF02469 - Fasciclin domain (Fasciclin) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGAGGCGAGGGAGGGGCGGCGCCGCATCA
Internal bar code:CACTACTGACGCTCCGGGCAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:964
LEAP-Seq percent confirming:95.4113
LEAP-Seq n confirming:71880
LEAP-Seq n nonconfirming:3457
LEAP-Seq n unique pos:115

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCAAGCAAAGGTGCGAGT
Suggested primer 2:GCCACCGTGTTATAGGAGGA