Insertion junction: LMJ.RY0402.168844_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.168844
Chromosome:chromosome 1
Location:2181192
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g011901 ARS7 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTTCCCGACAGGCCCGTGAAGTAGCCGG
Internal bar code:GTCCGCCATCCAGAGAATGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:903
LEAP-Seq percent confirming:99.745
LEAP-Seq n confirming:2738
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TGCATCTAGGCAGTTGATCG
Suggested primer 2:CAACTTCATTGTGTCCACGG