| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.168863 |
| Chromosome: | chromosome 1 |
| Location: | 567511 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003300 | (1 of 2) PTHR30483 - LEUCINE-SPECIFIC-BINDING PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGGGAAGCGCATGCAGCAGACTGCTGC |
| Internal bar code: | CTGTATATAGTTATCGCGTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 900 |
| LEAP-Seq percent confirming: | 99.657 |
| LEAP-Seq n confirming: | 9008 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGCTCAGAAGGACTTGC |
| Suggested primer 2: | TTGACCTGCTGCTTGTTTTG |