Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.168864 |
Chromosome: | chromosome 17 |
Location: | 2975135 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g720250 | LHCB4,CP29 | Chlorophyll a/b binding protein of photosystem II | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGGTTTGTGTGAGCGGAGGTTATCCCC |
Internal bar code: | GAAAGATATTTGCAACCCTACA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 757 |
LEAP-Seq percent confirming: | 99.7097 |
LEAP-Seq n confirming: | 6182 |
LEAP-Seq n nonconfirming: | 18 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGTGTTCTTGTCCAGAGCG |
Suggested primer 2: | TCGATGTAATCTCGCTGACG |