Insertion junction: LMJ.RY0402.168864_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.168864
Chromosome:chromosome 17
Location:2975153
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g720250 LHCB4,CP29 Chlorophyll a/b binding protein of photosystem II 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAGCAGTTGGCAACTTATCATACACAACA
Internal bar code:CTTCAGCGGGATTTGGCCGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:547
LEAP-Seq percent confirming:98.9376
LEAP-Seq n confirming:1490
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTGTTCTTGTCCAGAGCG
Suggested primer 2:TCGATGTAATCTCGCTGACG