| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168875 |
| Chromosome: | chromosome 12 |
| Location: | 3968693 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516600 | STK25 | (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED; Serine/threonine protein kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGGTCGCCCACGTGGACGGCGCCTGCA |
| Internal bar code: | AGAGAACATCATGGTGGACAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 616 |
| LEAP-Seq percent confirming: | 98.5021 |
| LEAP-Seq n confirming: | 3288 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCCTGTCCAACTGTGTG |
| Suggested primer 2: | AAGACACGGCTGGAGAACTG |