| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168880 |
| Chromosome: | chromosome 7 |
| Location: | 397911 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314900 | HEL34 | (1 of 1) PTHR24031:SF301 - ATP-DEPENDENT RNA HELICASE DDX18; DEAD/DEAH box helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTAATGCTTCTGCTCGGTCATACGCCAG |
| Internal bar code: | CCGGATCCTATCTACCCTAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 99.5745 |
| LEAP-Seq n confirming: | 936 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTATGGGAGACATGCGTTT |
| Suggested primer 2: | CTACAACAGCCACAGCCTGA |