Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.168891 |
Chromosome: | chromosome_12 |
Location: | 4417089 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g520950 | BOP5,DIC4,IC138 | Flagellar inner dynein arm I1 intermediate chain IC138 | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCGAAGGCCTTCACACGACTTTCTTATCGC |
Internal bar code: | CAGTGGGCTATCACGATTGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 928 |
LEAP-Seq percent confirming: | 99.1606 |
LEAP-Seq n confirming: | 2717 |
LEAP-Seq n nonconfirming: | 23 |
LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TAGTCATAGTCCGCCTCGCT |
Suggested primer 2: | ACGCCAGAAAGAGGACTTGA |