| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168891 |
| Chromosome: | chromosome 12 |
| Location: | 4417122 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520950 | BOP5,IDA-IC138,IC138,DIC4 | Axonemal inner dynein arm I1 intermediate chain; (1 of 1) PTHR12442:SF12 - WD REPEAT-CONTAINING PROTEIN 78 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTGGTACTTGCAGTGTAACGTTTGTGCA |
| Internal bar code: | TAATTCGCGGTCGCTTCGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1011 |
| LEAP-Seq percent confirming: | 99.3802 |
| LEAP-Seq n confirming: | 2405 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTCATAGTCCGCCTCGCT |
| Suggested primer 2: | ACGCCAGAAAGAGGACTTGA |