| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.168902 |
| Chromosome: | chromosome 13 |
| Location: | 4113355 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591550 | SRR20 | Scavenger receptor cysteine rich (SRCR) protein; (1 of 1) PTHR19331//PTHR19331:SF279 - LYSYL OXIDASE-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGACTGGAAATGCTTCACGAACCTTAC |
| Internal bar code: | CACGAAAGCTCCCTCGATGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 832 |
| LEAP-Seq percent confirming: | 99.7553 |
| LEAP-Seq n confirming: | 6115 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGATAATATTAACGGCGG |
| Suggested primer 2: | CTAGGTCTTCCGCAACAAGC |