| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.168910 |
| Chromosome: | chromosome 10 |
| Location: | 1301818 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g427453 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGCGAAACAGGGTGCAGGTTAGCTGGG |
| Internal bar code: | TTCCTGTTCGTGTATCTAAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 962 |
| LEAP-Seq percent confirming: | 74.0943 |
| LEAP-Seq n confirming: | 1084 |
| LEAP-Seq n nonconfirming: | 379 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATCAGGCGGCTATACATT |
| Suggested primer 2: | CGAGACAGCTGCTACTGTGG |