| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.168944 |
| Chromosome: | chromosome 7 |
| Location: | 566775 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g316526 | (1 of 8) 3.1.1.13//3.1.1.3 - Sterol esterase / Triterpenol esterase // Triacylglycerol lipase / Triglyceride lipase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTAGCCCAAAAGGGTCCGGCTCGGCGCCG |
| Internal bar code: | GTGACGGGGCATGGGATCCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 381 |
| LEAP-Seq percent confirming: | 99.0099 |
| LEAP-Seq n confirming: | 200 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTTTCGTGCCTCCAGTC |
| Suggested primer 2: | CCTATCCTGGATGACGTGCT |