| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.169002 |
| Chromosome: | chromosome 2 |
| Location: | 8694410 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g142186 | FTSZ2 | Plastid division protein FtsZ2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAGCGTGGCAATGGATATGCCCGACGCA |
| Internal bar code: | GGACAGCCCGCCGAATTGTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 265 |
| LEAP-Seq percent confirming: | 99.904 |
| LEAP-Seq n confirming: | 1041 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGTTGGTGTGAGCTTTCG |
| Suggested primer 2: | CGGGACTATTTGGCTTGGTA |