| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.169003 |
| Chromosome: | chromosome 2 |
| Location: | 1490586 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g084050 | SEC62 | SEC62-subunit of ER-translocon; (1 of 1) K12275 - translocation protein SEC62 (SEC62) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGACGGCGGGGGTGACCTGGGGCGGAG |
| Internal bar code: | GGGTTGTCCAGTGAGCAGTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 367 |
| LEAP-Seq percent confirming: | 99.8905 |
| LEAP-Seq n confirming: | 4562 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTAGGCCTGGAGACTGAG |
| Suggested primer 2: | CTCGACTATCTGGTGCGTGA |