| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.169006 |
| Chromosome: | chromosome 6 |
| Location: | 2967798 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273200 | (1 of 1) K03762 - MFS transporter, MHS family, proline/betaine transporter (proP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCATGTGTGAATCATGCGCTCACGCTTT |
| Internal bar code: | CTCTCCGTAGCTCTCAGCCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 975 |
| LEAP-Seq percent confirming: | 99.521 |
| LEAP-Seq n confirming: | 6441 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACATGTGGAACACACAC |
| Suggested primer 2: | CTCGGAAGCAGGTCAAGTTC |