| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.169028 |
| Chromosome: | chromosome 4 |
| Location: | 3703783 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g229163 | (1 of 1) PF00169//PF02181 - PH domain (PH) // Formin Homology 2 Domain (FH2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCCACGCCTCCACGCTACCAAAACGGGA |
| Internal bar code: | GAAGGTGTGGGTCGGGCAACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 8 |
| LEAP-Seq percent confirming: | 72.1264 |
| LEAP-Seq n confirming: | 251 |
| LEAP-Seq n nonconfirming: | 97 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAGCTGTCAATGTGAAGA |
| Suggested primer 2: | TCGTCGCAAGCAACTAGAGA |