| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.169033 |
| Chromosome: | chromosome 12 |
| Location: | 103627 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g484150 | (1 of 1) K15501 - serine/threonine-protein phosphatase 6 regulatory subunit 3 (PPP6R3, SAPS3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGCCCACACAGTTCCTGGAGTACACGT |
| Internal bar code: | AATGACTCCAGGACGGCCGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1148 |
| LEAP-Seq percent confirming: | 99.3627 |
| LEAP-Seq n confirming: | 4054 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGGCAGGTGTAGCAGGA |
| Suggested primer 2: | CAGCTCTGGACTAGGGCATC |