| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.169037 |
| Chromosome: | chromosome 10 |
| Location: | 102008 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418400 | (1 of 2) PF08590 - Domain of unknown function (DUF1771) (DUF1771) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTGGTTGAGTAGGAAGTACCATCGGCG |
| Internal bar code: | GTTGGACCTGGTGGTGCACTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 261 |
| LEAP-Seq percent confirming: | 99.6723 |
| LEAP-Seq n confirming: | 1825 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGGTTGAAACGGGTCAG |
| Suggested primer 2: | TAATGACCGCCTAAACTGCC |