| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.169049 |
| Chromosome: | chromosome 17 |
| Location: | 6273624 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g742450 | GST15 | (1 of 6) K00799 - glutathione S-transferase (GST, gst); Similar to Glutathione S-Transferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATACGGTACTCACCACTTCTTTCATCTTG |
| Internal bar code: | ATAGCTGCGTTATGCTAGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1137 |
| LEAP-Seq percent confirming: | 99.1982 |
| LEAP-Seq n confirming: | 4825 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGGTACGGTGGAGTGTG |
| Suggested primer 2: | ACTTTCATCACACAGCACGC |