| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.169049 |
| Chromosome: | chromosome 17 |
| Location: | 6273631 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g742450 | GST15 | (1 of 6) K00799 - glutathione S-transferase (GST, gst); Similar to Glutathione S-Transferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGAGTAAGGCAGTAGGCTGCGAAGGGA |
| Internal bar code: | GGGAACGGCAAGGTTCAAGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 761 |
| LEAP-Seq percent confirming: | 82.6057 |
| LEAP-Seq n confirming: | 6036 |
| LEAP-Seq n nonconfirming: | 1271 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGGTACGGTGGAGTGTG |
| Suggested primer 2: | ACTTTCATCACACAGCACGC |