| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.169051 |
| Chromosome: | chromosome 1 |
| Location: | 6978843 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050400 | TIM22C | Mitochondrial inner membrane translocase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACATGGCGCAGGGCGGTGTGAGGGAGGC |
| Internal bar code: | CCTATGGGCCTGGGCGCGTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 510 |
| LEAP-Seq percent confirming: | 99.5379 |
| LEAP-Seq n confirming: | 7109 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCATCTTTACACAGCGCCA |
| Suggested primer 2: | TCCTGGGTCTTTGACTTTGG |