Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.169059 |
Chromosome: | chromosome_7 |
Location: | 3992020 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g339850 | sense | intron|outside_mRNA |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGCTGTTCGTACTGGGGCTTAGGCATTGGC |
Internal bar code: | GGTAACCTTGTCGGTGCATCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 620 |
LEAP-Seq percent confirming: | 93.5854 |
LEAP-Seq n confirming: | 1386 |
LEAP-Seq n nonconfirming: | 95 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGGTTGTAGCAGAGCTGGG |
Suggested primer 2: | GGCACCAGTCTTAAGCAAGC |