Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.169068 |
Chromosome: | chromosome_4 |
Location: | 335520 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g217908 | FAP79 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGCTGGGCAAGAGCGCCAGTGTCAACGCT |
Internal bar code: | GTGTGCGCGATTAATTCAGTGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 935 |
LEAP-Seq percent confirming: | 99.6562 |
LEAP-Seq n confirming: | 1739 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACATACCGCACGAAAGAACC |
Suggested primer 2: | CACTCGGCAAGCTATGTTCA |